#Bio rad western blot all blue standards plus#

No conjugate is needed for fluorescent blot detection Read More. Fluorescent detection: prepare a 1:10 dilution of the WesternC Standard and load 5l per well.

#Bio rad western blot all blue standards plus#

That's why we're pleased to launch the color-enhanced Blu10 Plus (BLUltra) prestained protein standards.Īs you may see the comparison data shown below, Blu10 Plus (BLUltra) shows a better resolution than the Blu12 (BLUeye) & Blu10 (BlueRAY) for the protein bands from 95 kDa to 270 kDa. Loading buffer: 30 (w/v) glycerol, 2 SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02 NaN 3, 0.01 bromophenol blue. Nowadays, more and more researchers are studying the high molecular weight proteins, meaning they may need high-resolution prestained protein standards, especially with high molecular weights. These systems consist of a darkroom hood, CCD camera, and software-controlled motorized lens, UV and white light illuminators, filter slider with a standard. Additional data should be considered for a more accurate adjustment. Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. 2.5 μl per well for general Western transferring. Migration patterns of Blu10 Plus Prestained Protein Ladder (BLUltra) in different electrophoresis conditions are listed below :ģ μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. Guide for Molecular Weight Estimation (kDa) The Blu10 Plus Prestained Protein Ladder (BLUltra) keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes. These standards can be used for fluorescent blot detection at red wavelengths (635 nm laser or red LED). The 10 recombinant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. Precision Plus Protein All Blue Standards are a mixture of ten blue-stained recombinant proteins (10250 kD), including three reference bands (25, 50, and 75 kD). Improved Dual Color Standards are brighter than the competition on both gels and blots.The Blu10 Plus Prestained Protein Ladder (BLUltra) is a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa. Protein standards for western blotting applications selection guide. Recombinant Precision Plus Protein Standards. Increased overall brightness provides visual robustness throughout the entire protein electrophoresis and western blotting workflow. Bio-Rads protein standards for western blotting applications. 8 blue-stained bands and 2 pink reference bands (25 and 75 kD) Dilute all required primary antibodies to the required.Perform gel electrophoresis using a suitable protocol. Mixture of 10 recombinant proteins of precise molecular weights (10–250 kD) weight marker such as Bio-Rad’s Precision Plus All Blue Standards (161-0373) alongside your samples to determine the molecular weight of any proteins you visualize at the end of the blotting process.Use improved Precision Plus Protein Dual Color Standards for molecular weight estimation on SDS-PAGE gels and western blots. Protein molecular weight markers, sometimes referred to as protein standards or protein ladders, are used to estimate the molecular weight of proteins of interest and to monitor the progress of electrophoretic separation or transfer in Western blotting. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. Life Science Web site USA 800 4BIORAD Australia 61 02 9914 2800 Austria 01 877 89 01. LC-MS innovation: Improve analytical accuracy 161-0373 Precision Plus Protein All Blue Standards. Which microbioreactor modules are best for you Which solid support is right for your oligo synthesis? Lab Automation, AI & Digital Transformation.Food Contamination: Quality, Safety & Authenticity.